Granulocyte Colonies Derived from Lymphocyte Fractions of Normal Human Peripheral Blood

Granulocyte colonies derived from lymphocyte fractions of normal human peripheral blood.

Granulocyte colonies can be grown from leukocyte-rich plasma obtained from normal human peripheral blood. Purification of lymphocytes by the Ficoll-Hypaque gradient method and subsequent removal of monocytes achieved a 10-fold increase in the number of colonies formed, suggesting that the colony-forming cell is nonadherent, nonphagocytic, and morphologically difficult to distinguish from a lymp...

Ultrastructural Analysis of Hematopoietic Colonies Derived from Human Peripheral Blood

The ultrastructure of granulocyte colonies derived from normal human peripheral blood leukocytes cultured in semisolid media has been studied by a new method developed for this purpose. Fixation, dehydration, and embedding of the whole content of the Petri dish resulted in a block of Epon containing colonies made up of cells with the spatial orientation of those observed in living cultures. Thi...

Gene Expression Profile of Adherent Cells Derived From Human Peripheral Blood: Evidence of Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) provide a novel option in cellular therapy and tissue engineering. Recent studies indicated that it is possible to obtain MSCs from peripheral blood by attachment ability to plastic surface. To evaluate adherent cells derived from peripheral blood, their expression profile and surface markers were investigated. The results of RT-PCR indicated that these cells expre...

Characteristics of endotoxin altering fractions derived from normal human serum.

Mononuclear Cells Normal Human Peripheral Blood Myeloid-Derived Suppressor Cells from Characterization of Cytokine-Induced